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Scion feeder 3 mm free download
By using our site, you agree to our collection of information through the use of cookies. To learn more, view our Privacy Policy. To browse Academia. Log in with Facebook Log in with Google. Remember me on this computer. Enter the email address you signed up with and we’ll email you a reset link. Ссылка на страницу an account? Click here to sign up. Download Free PDF. Protoplast isolation and culture for banana regeneration via somatic embryogenesis Fruits, A short summary of this paper.
PDF Pack. People also downloaded these PDFs. People also downloaded these free PDFs. Plant regeneration from cultured protoplasts of the cooking banana cv. Bluggoe Musa spp. Sihachakr and R. Scion feeder 3 mm free download simplified protocol to induce callogenesis in protoplasts of date palm Phoenix dactyliferaL. Plant regeneration from indica rice Oryza sativa L. Plant regeneration from protoplasts of mango Mangifera indica L. Isolation of Protoplasts Banana Musa acuminate Scion feeder 3 mm free download cvs.
Plant regeneration from embryogenic cell suspensions and protoplasts in sugarcane Saccharum spp. CoL by Javed Iqbal. Current Biotechnology, Mehpara, by Abdul Mujib. Download Download PDF. 10 professional to home free PDF.
This protocol describes a method for obtaining protoplasts from robert. The principle, key advantages, starting 2 Univ. Guelph, Dep. Plant plant material, time required and expected results are presented. Materials and methods. The first protoplasts may be seen after 30 min of incubation Ontario, Canada in enzyme maceration. With protoplasts from embryogenic cell suspension, complete devel- 3 Lab. The Nguyen Van Cu, Q.
The transfer of derived embryo plantlets, at 8—10 weeks after protoplast plating, onto growth regulator-free medium, 4 leads to plant rooting and elongation. Rabat, Univ. DOI: Introduction still yellow and rolled, are suitable for protoplasts allowing morphogenetic devel- opment.
Applications Banana protoplasts may be used for Time required research purposes in physiology and phy- scion feeder 3 mm free download, and for banana breeding, Four to five hours are required for media including genetic transformation, through preparation; 10—14 h for enzymatic treat- electroporation or PEG and somatic hybrid- ment; 3 h scion feeder 3 mm free download protoplast purification, stain- isation [1—3]. After maceration, protoplasts are sep- arated from debris by sieving [4].
Starting material [8, 9] 2. Materials and methods The protocol requires: 2. Laboratory equipment — an embryogenic banana cell suspension, The protocol requires: Note: better results are obtained using embryogenic cell suspension 3—4 days after — a culture room illuminated 12 h a day at the last subculture.
Meso-inositol — Preparation of the enzymes Nicotinic acid — 2. Prepare, centrifuge and filter. Enzyme solution EC2 for protoplast Enzyme solution EC3 for protoplast isolation from banana calli isolation from in vitro banana leaves — Add to distilled water: 1. Table II. Prepare, centrifuge and sterilise as for the N6 liquid culture medium required for banana protoplast culture. Adjust to pH 5. Boric acid H3BO4 1. Adjust to Ascorbic acid — 1 pH 5. Choline chloride — 1 Note: for preliminary experiments S2 B12 — 0.
Adjust it to pH 5. Mannitol — Organic acids N6 liquid culture media for protoplast Na pyruvate — 5 culture Citric acid — 10 Malic acid — 10 Prepare a N6 free 1 pack civil autocad autodesk service 3d 2015 culture media [10] Fumaric acid — 10 table II.
Vitamins A — 0. Adjust the medium to pH 5. Prepare a regulator-free MS medium with Continued. Zeatine — 0. Note: for PCV cell manipulations, the point of the pipette or cone has to be cut to enlarge the section. Preparation of feeder layer system [12] — Solubilise 1. Cover with a sterilised nitrocellulose filter and seal with plastic film.
Note: feeder scion feeder 3 mm free download may be keep at room temperature for 1—5 days before use. Protoplast isolation Protoplast isolation from cell scion feeder 3 mm free download — Use embryogenic suspension cultures 3— 4 days after the last subculture, as donor — Sieve the cell suspensions through a ster- Figure 1.
Fruits, vol. Figure 2. Collect Figure 3. After calcofluor staining, cell wall degradation can be visu- alised, through a UV microscope, on sam- ples of protoplast suspension. Protoplast viability is determined using fluoresceine diacetate. Protoplast yield is estimated using a Nageotte hematocytometer. Petri dish containing feeder layer culture — Mix gently.
Controls on cell wall 30 min of incubation. The first cell divisions occur on feeder layers from 3—8 days after protoplast plating. Pro- Protoplast isolation from banana leaves: embryo formation is observed 14—21 days — In a small Petri dish, weigh 1 g of very after initiation of protoplast culture.
Weekly young leaves; add 4 mL of EC3 solution. Protoplast regeneration through somatic embryogenesis Three weeks after protoplast plating, pro- embryos are individually picked up from the feeder layer and gently transferred onto regeneration medium.
In vitro and soil plant development Eight to ten weeks after protoplast plating, transfer derived embryo plantlets onto growth regulator-free medium for rooting and elongation.
After reaching the Figure 4. Troubleshooting Seven main problems can occur: a Protoplast yield from cell suspension is low because the suspension quality is not good enough. Figure 5. Solutions: regularly subculture cell suspen- Banana protoplast sion every 6 days before protoplast extrac- development on feeder layer system. Solutions: Use only the very young part of growing calli, and only 1—2-cm-long imma- ture leaves.
Solutions: check with calcofluor under a UV microscope the eventual presence of the cell wall. Figure 7. The transfer of feeder layer system.
References [1] Sihachakr D. Solutions: check the embryogenic potential of cell suspension. If protoplasts brown, [4] Assani A. Figure 8. Solutions: check the sterility of each viability of cultured cells, Stain Technol.
Typical results obtained plasts of the cooking banana cv. Wehr B. The first cell divisions occur on feeder layers Ambroise A. Proembryo formation is eration in banana and plantain cultivars Fruits, vol. Demarly Y. Rose protoplast isolation and culture and heterokaryon selection by immobilization in extra thin alginate film by Pratap Pati. Somatic embryogenesis in protoplast derived calli of cultivated jute, Corchorus capsularis L by Saha Thakurdas. How to regenerate plantlets from protoplasts of Fritillaria imperialis by Esmaeil chamani.
Ссылка на подробности protocol for protoplast isolation and plant regeneration scion feeder 3 mm free download Fritillaria imperialis L by Esmaeil chamani.